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一.產品名稱：Recombinant Human PDGF-BB/Peprotech PDGF-BB

二.Catalog Number:AF-100-14B

三.Synonyms:

Plaet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

四.Description:

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in plaet α-granules and are released upon plaet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids). . Manufactured using all non-animal reagents.

五.AA Sequence:

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED₅₀ for this effect is 1.0-3.0 ng/ml.

九.Cross Reactivity:Cow, Monkey, Mouse, Pig, Rat, Human